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D whether IGFBP3 modulates GIST cell response to imatinib, we first sought to determine whatPage 2 of(page number not for citation purposes)Molecular Cancer 2009, 8:http://www.molecular-cancer.com/content/8/1/GIST882 KIT exon 13: K642EGIST-T1 KIT exon 11: V560delYADBImatinib IGFBP3 -tubulin24 hr48 hrEImatinib IGFBP3 -tubulin24 hr48 hr-+-+-+-+3 BP F IG rhCFigure 1 Imatinib-induced IGFBP3 expression
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D whether IGFBP3 modulates GIST cell response to imatinib, we first sought to determine whatPage 2 of(page number not for citation purposes)Molecular Cancer 2009, 8:http://www.molecular-cancer.com/content/8/1/GIST882 KIT exon 13: K642EGIST-T1 KIT exon 11: V560delYADBImatinib IGFBP3 -tubulin24 hr48 hrEImatinib IGFBP3 -tubulin24 hr48 hr-+-+-+-+3 BP F IG rhCFigure 1 Imatinib-induced IGFBP3 expression
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Expression is lost in many cancer cells [44-46], and reintroduction of the protein often results in cell death [21,46,47]. Similarly, our results show that IGFBP3 expression is not detectable in GIST-T1 cells but overexpression leads to loss of cell viability. Indeed, the growth inhibitory and pro-apoptotic effects of IGFBP3 are well established in a variety of in vitro and in vivo cancer models.
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