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Nally substitute for Roc2. We tested this by introducing a Roc1a transgene into the Roc2KG mutant background. Even in this genotype, Roc1a bound to Cul1 but not detectably to Cul5 (Fig. 8). Moreover, as we show in Fig. 6G, the pool of Cul5 available for binding Roc1a is greatly reduced in Roc2 mutant animals relative to wild type. Thus, the Cul1 and Cul5 E3 ligase complexes form independently of o
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